It’s August Technical Advances update time! See the newly added papers here FlyBase:Papers with technical advances - FlyBase Wiki. Don’t forget to flag when you Fast-Track Your Paper if you want your paper with a new technique, reagent, or resource featured next month.
Generation of six4 -nlsRFP: a red somatic gonadal nuclear marker for live imaging Drosophila gonadogenesis. (MicroPubl Biol)
Reagent to detect somatic gonadal cell nuclei with RFP during embryogenesis
Insulator BEAF32 regulates expression of tissue-specific genes and piRNA source loci in Drosophila ovaries. (Epigenetics Chromatin)
We generated a heatmap with hierarchical clustering of the differentially expressed Drosophila gene groups which we called Anatomy Gene expression in Fly (AnGeFly) and created a web-based application [https://akob.shinyapps.io/angefly/]
A deep learning model for accurate segmentation of the Drosophila melanogaster brain from Micro-CT imaging. (Dev. Biol.)
We generated a deep learning AI model for automated segmentation of the adult fly brain from microcomputed tomography imaging. This model is freely available and can be adapted to individual user needs.
Deep learning-based high-resolution time inference for deciphering dynamic gene regulation from fixed embryos. (Nat. Commun.)
We developed a deep learning algorithm to infer the developmental time from fixed Drosophila embryo images (nc11-early nc14) at 1-minute resolution, without the need for live imaging.
Growth phase diets diminish histone acetyltransferase Gcn5 function and shorten lifespan of Drosophila males. (EMBO Rep.)
live yeast-fly assay: We imposed dietary interventions throughout the entire larval stages using the KO collection of yeast, and then quantified the lifespans of eclosed adults on the common standard food to assess how the larval diets affect the adult health.
Nonmuscle myosin II regulates presynaptic actin and neuronal mechanobiology in Drosophila. (J. Cell Biol.)
QUAS-mScarlet-I::Act5C, QUAS-Tm1-A::mNeonGreen, QUAS-Tm1-L::mNeonGreen, QUAS-mNeonGreen::GMA
Evolution of a novel left-right asymmetry in organ size by co-option of a tissue rotation process. (Development)
Membrane EYFP marker pB-act5C::DE-Cad-EYFP_2: shg 5’UTR, first two cadherin repeats, trans-membrane domain, part of the cytoplasmic domain without the beta-Catenin binding domain, inserted into pBAC-ECFP-15xQUAS_TATA-mcd8-GFP-SV40 (Addgene 104878) with integration marker 3xP3 DsRed.
High-throughput measurement of Drosophila feeding behavior. (Cell Rep Methods)
FlyPlate-BCA provides a high-throughput, accurate method to quantify food intake in Drosophila via BCA protein assay, improving sensitivity and reducing sample requirements for diet and aging research.
Dying occurs as a defined molecular progression in Drosophila rather than as nonspecific physiological collapse. (iScience)
Pseudotime analysis of single-fly transcriptomic data to overcome population heterogeneity in analysis of a phenotype
Temperature-dependent modulation of light-induced circadian responses in Drosophila melanogaster. (EMBO J.)
Monitoring the calcium activity of neurons in real-time under a two-photon microscopy in live Drosophila, which can be coupled with optogenetics.
Endogenous OptoRhoGEFs reveal biophysical principles of epithelial tissue furrowing. (Nat. Commun.)
endogenously-tagged optogenetic RhoGEFs