bad unc-119 rescue due to peel-1?

Model Organism: Worms Methods & Reagents
1

Something strange happened with our recent injections for genome engineering: the F1s with arrays (based on the presence of RFP from the co-injection markers) were very poorly rescued for unc-119. The reason we are wondering if this effect might be due to the Phsp-16.41::peel-1 plasmid is that two people with completely different injection components had the same poor rescue of unc-119, whereas in previous injections for Mossci/del where we didn’t use peel-1 (and just relied on the absence of RFP to identify integrants), the unc-119 was fully rescued. I injected the peel-1 at 10 ng/ul. I’d appreciate any thoughts.
Thanks,

Claire

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Hi Claire,
For what it is worth, we have not noticed what you describe here in Erik’s lab after including the hsp:peel-1 construct in the injection mix. We get good rescue of the unc-119 phenotype in injection mixes that contain the peel-1 construct. Any chance you might inadvertantly be activating the heat-shock response before the actual temperature change so you’re selecting against arrays in general?

Christian