We just now created a transgenic strain using Biolistic transformation.
We were able to rescue the unc phenotype and we can even see the presence of GFP.
To confirm the presence of the fosmid construct in this transgenic strain, We did genotyping along with the positive control (Fosmid construct).
We only saw the band in the positive control not the transgenic strain which we created.
Has anyone had this problem before? Is there any way I can troubleshoot?