Bleach vs. Egg Lay are there differences?

Has anybody ever done a side by side lifespan or transcriptomics experiment with N2s that were started from an egg lay and compared them to worms that were started from a bleach prep?


I recently performed the lifespan assays with worms synced by egg-laying and bleach prep methods. I have not seen any difference in WT and some mutants I work with. Make sure you are picking the worms at the right stage in both assays (either L4 or YA base don your experiment).