When : April 12 - 25, 2023
Where: Cold Spring Harbor Labs, New York
registration: Cell & Developmental Biology of Xenopus: Gene Discovery & Disease 2023 | CSHL
Application Deadline: January 31, 2023
Arrival: April 11th by 6pm EST
Departure: April 25th around 12pm EST
Due to the tailored nature of this course, it is suitable for those new to the Xenopus field, as well as for more advanced students who are interested in emerging technologies.
During the course, the students will analyze any phenotypes generated from CRISPR/Cas9 based gene depletion while learning the diverse array of techniques available in Xenopus. Approaches covered will include microinjection and molecular manipulations such as CRISPR/Cas9 knockouts, antisense morpholino-based depletions, transgenics, and mRNA overexpression. In addition, students can combine these techniques with explant and transplant methods to simplify or test tissue level interactions, mRNA in situ hybridization and protein immunohistochemistry, basic bioinformatic techniques for gene comparison and functional analysis. Biochemical approaches such as proteomics and mass spectrometry and biomechanical concepts will also be discussed and we will introduce a variety of sample preparation and imaging methods including time-lapse, fluorescent imaging, optical coherence tomography and confocal microscopy.
Organizers: Chenbei Chang, University of Alabama at Birmingham, and Lance Davidson, University of Pittsburgh