Has anyone used cholesterol from Alfa Aesar? It is 96% rather than 99% pure cholesterol (3% “other steroids”). We ordered it in lieu of the usual Sigma-Aldrich because Sigma did not have it in stock for a couple of months. GMW is same as Sigma’s, but temp to store is RT, not -20C. How critical is it that the cholesterol be 99% pure for NGM plates?

Thank you,


we get our cholesterol from a local supplier, it’s only >=95%, we store it @ 4C and the worms grow fine…you can relax.

I guess if you were heavily into lipid metabolism or dauermone production research you might be more picky.


Great, thank you!