| Allele | Deleted ORF? | Reverts? | Notes | Molecular Descriptiona | Reference |
|---|---|---|---|---|---|
| ade1-14 | no | yes | red colonies | TGG-to-TGA nonsense change at codon 244; GGA-to-GAA missense change at codon 185 | Nakayashiki et al. 2001 |
| ade1-101 | no | simple point mutation causing a requirement for adenine | Botstein et al. 1979 | ||
| ade2-1 | no | yes | ochre mutation | TTA-to-TTG silent change at codon 9, GAA-to-TAA ochre nonsense change at codon 64, AGA-to-GGA missense change at codon 101, GTT-to-GTC silent change at codon 124, ACG-to-ACA silent change at codon 539 | Rodney Rothstein, Personal communication to SGD |
| ade2-101 | no | yes | ochre mutation, red colonies | G to T transversion at nucleotide 190, changing codon 64 from a Glu to a Stop | Gai and Voytas, 2005 |
| ade2-BglII | no | no | red colonies | frameshift (BglII site filled in at position 592) | Engebrecht and Roeder 1990 |
| can1-100 | no | yes | ochre mutation | AAA-to-TAA ochre nonsense change at codon 47 | Rodney Rothstein, Personal communication to SGD |
| his3delta200 | yes | no | Cold sensitive; high frequency of petite formation, especially during transformation. Note that this deletion damages the PET56 promoter. See Zhang et al. (2003) for a discussion of this issue. | 1 kb deletion (-205 to 835) | Struhl 1985; Fasullo and Davis 1988 |
| his3delta1 | partial | no | chemically constructed deletion; reversion is undetectable (< 10(E-9) revertants in a culture). Causes histidine to be required for growth; allows selection of vectors carrying the HIS3 gene. | 187 bp HindIII-HindIII internal deletion (305 to 492) | Scherer and Davis 1979 Botstein et al. 1979 |
| his3-11,15 | no | no | double mutant | G deletion at nucleotide 208, G deletion at nucleotide 319 | Rodney Rothstein, Personal communication to SGD |
| his3delta | yes | no | In the S288C background, the his3 null mutation confers sensitivity to acetic acid. | complete deletion | Ding et al. 2013 |
| leu2delta1 | partial | no | EcoRI-ClaI internal deletion (163 to 649, 0.6 kb) | Sikorski and Hieter 1989 | |
| leu2-3,112 | no | no | double mutant; leu2-3 and leu2-112 are each frameshift mutations; the double mutants revert very infrequently (less than 10(E-8) revertants in a culture) and cause leucine to be required for growth. This allows the selection of vectors carrying the LEU2 gene. | GTC-to-GTT silent change at codon 56, GTT-to-GCT missense change at codon 69, G insertion at nucleotide 249, G insertion at nucleotide 792, GTT-to-GTC silent change at codon 299, GAC-to-AAC missense change at codon 300 | Hinnen et al. 1978;Gaber and Culbertson 1982; Meira LB et al., 1995; Rodney Rothstein, Personal communication to SGD Botstein et al. 1979 |
| leu2delta | yes | no | In the S288C background, the leu2 null mutation confers sensitivity to acetic acid. | complete deletion | Ding et al. 2013 |
| lys2-801 | no | yes | amber mutation | ||
| lys2delta202 | partial | no | XhoI-HpaI internal deletion (1813 to 2864, 1.0 kb) | Winston et al. 1995 | |
| lys2delta | yes | no | In the S288C background, the lys2 null mutation confers sensitivity to acetic acid. | complete deletion | Ding et al. 2013 |
| trp1delta1 | yes | no | cold sensitiveb, weak galactose inducer (deletes GAL3 UAS), removes ARS1, also called trp1-901 | 1.45 kb deletion, EcoRI-EcoRI (-102 to 1352) | Sikorski and Hieter 1989 |
| trp1delta63 | partial | no | cold sensitiveb | 0.6 kb deletion, EcoRI-HindIII (-102 to 513) | Sikorski and Hieter 1989 |
| trp1-1 | no | yes | amber mutation | GAG-to-TAG amber nonsense change at codon 83 | McDonald et al. 1997 |
| trp1-1 | no | yes | cold sensitiveb | C to T at residue 403 of the coding sequence, changing residue 135 from glutamine to an amber stop codon | Brian Green and Joachim Li, unpublished results |
| trp1delta | yes | no | In the S288C background, the trp1 null mutation confers sensitivity to acetic acid. | complete deletion | Ding et al. 2013 |
| trp1-289 | no | yes | Reverts at low frequency (about 10(E-7) revertants in a culture) and causes tryptophan to be required for growth. | Allows the selection of vectors carrying the TRP1 gene. | Botstein et al. 1979 |
| trp5delta | yes | no | In the S288C background, the trp5 null mutation confers sensitivity to acetic acid. | complete deletion | Ding et al. 2013 |
| ura3-52 | no | no | Ty1 insertion (transcribing left to right) at pos. 121 | Rose and Winston 1984 | |
| ura3-1 | no | yes | G to A transition at residue 701 of the coding sequence, changing residue 234 from glycine to glutamate | Yan Li, Glenn Manthey, and Adam Bailis, unpublished results | |
| ura3delta | yes | no | In the S288C background, the ura3 null mutation confers sensitivity to acetic acid. | complete deletion | Ding et al. 2013 |
| ura4delta | yes | no | In the S288C background, the ura4 null mutation confers sensitivity to acetic acid. | complete deletion | Ding et al. 2013 |
The alleles listed below are described in Brachmann et al. 1998.
| Allele | Deleted ORF? | Reverts? | Notes | Molecular Descriptiona | Reference |
|---|---|---|---|---|---|
| ade2delta::hisG | no | no | (Aparicio et al. 1991) | ||
| leu2delta0 | yes | no | designer deletion | Brachmann et al. 1998 | |
| lys2delta0 | yes | no | designer deletion | Brachmann et al. 1998 | |
| met15delta0 | yes | no | designer deletion | Brachmann et al. 1998 | |
| ura3delta0 | yes | no | designer deletion | Brachmann et al. 1998 | |
aThe sequence coordinates are relative to the first ATG of the selectable marker ORF, in which the A residue is defined as +1.
bAll trp- strains are cold sensitive (Singh and Manney 1974).