Hi,
An undergraduate student wants to overexpress a gene spatiotemporally in different neurons after Day 1 adulthood. Would appreciate if anyone help us in working out the possible strategy here.
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Hi there,
I think you’d want to have a two-part system.
First: An inducible Cre or inducible Flippase (like by heatshock).
Second: A plasmid that has your student’s neuronal promoter::lox::STOP::lox::gene or FLP-on equivalent, and inject that as an array.
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Other options for a two-part system include tissue-specific rescue of the heat-shock response, split cGal, and Tet/Q.
I would recommend reading Nance and Frokjaer-Jensen’s review in Wormbook, especially the section on bipartite systems.
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Also see TET-ON system in:
Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange.
**Nonet ML.**Genetics. 2020 Aug;215(4):903-921. doi: 10.1534/genetics.120.303388. Epub 2020 Jun 8.PMID: 32513816 Free PMC article.
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