Confused : 1-day old adult hermaphrodite

Model Organism: Worms Methods & Reagents
1

Hi all,

i am confused by the term “1 day old hermaphrodite” in paper regarding infection in worm.
Is it referring to a “fresh” adult (which just turn into adult from L4), or it’s 24hours after the worm turn into adult? ???

Thanks…

2

Every time I’ve seen the term, I’m pretty sure it’s been used to mean the latter, to mean an animal roughly 24 hours after the L4-adult molt.

3

I agree with Hillel, every time I’ve seen that term it is to describe the latter.

4

.I agree with Hillel and Snug, that is what I think it means, 24hrs from when the worm turned into L4.

5

Tyrael,

From what I have seen, this term ‘1 (or one) day adult’ is used exclusively by Frederick Ausubel and in a couple of his papers he actually writes that worms were grown until they were 1 day adults rather than just stating one-day-adults…hence that means I guess 24 hours after the L4-adult molt.

If there is any remaining doubt ??? you could email Fred Ausubel and ask him.

ausubel@molbio.mgh.harvard.edu

Steve

6

It does mean 1 day after the L4 stage. It’s simpler to do it this way in ageing studies as some mutants develop slower than wild-type, some have strong dauer-inducing mutations, etc. People don’t tend to include the developmental period in ageing analyses

7

Thank you all for the prompt replies in helping to clear my doubts!! Appreciate it very much!

Sharp eye you have, stevenh! Yes, i am reading about the paper from Ausubel’s lab… Just that wondering is adult hermaphrodite is good to be used, since sometimes the bagging effect could confound the experiment.