Hi all,
i am confused by the term “1 day old hermaphrodite” in paper regarding infection in worm.
Is it referring to a “fresh” adult (which just turn into adult from L4), or it’s 24hours after the worm turn into adult? ???
Thanks…
Hi all,
i am confused by the term “1 day old hermaphrodite” in paper regarding infection in worm.
Is it referring to a “fresh” adult (which just turn into adult from L4), or it’s 24hours after the worm turn into adult? ???
Thanks…
Every time I’ve seen the term, I’m pretty sure it’s been used to mean the latter, to mean an animal roughly 24 hours after the L4-adult molt.
I agree with Hillel, every time I’ve seen that term it is to describe the latter.
.I agree with Hillel and Snug, that is what I think it means, 24hrs from when the worm turned into L4.
Tyrael,
From what I have seen, this term ‘1 (or one) day adult’ is used exclusively by Frederick Ausubel and in a couple of his papers he actually writes that worms were grown until they were 1 day adults rather than just stating one-day-adults…hence that means I guess 24 hours after the L4-adult molt.
If there is any remaining doubt ??? you could email Fred Ausubel and ask him.
ausubel@molbio.mgh.harvard.edu
Steve
It does mean 1 day after the L4 stage. It’s simpler to do it this way in ageing studies as some mutants develop slower than wild-type, some have strong dauer-inducing mutations, etc. People don’t tend to include the developmental period in ageing analyses
Thank you all for the prompt replies in helping to clear my doubts!! Appreciate it very much!
Sharp eye you have, stevenh! Yes, i am reading about the paper from Ausubel’s lab… Just that wondering is adult hermaphrodite is good to be used, since sometimes the bagging effect could confound the experiment.