Hi all,
Thanks for your help in advance. I just performed an EMS mutagenesis using several hundreds Po animals at an EMS concentration of 50mM (4mls of M9 gently rocking for 4hrs). At the end something like 95% of my worms were dead. Is that normal? They were all sticks…very discouraging.
Are these results typical? I am mutagenizing a mutant, but its not that sickly of a strain, so its confusing. I should mutagenize N2 as a control, but thought I’d ask the general community about this first.
Cheers…