Hi,
Does anyone have experience using calcein-AM (drinking assay) ? It is usually used to monitor the speed of food intake as a measure of pharyngeal pumping rate.
I would like to use it, however, as a quantitative measure of total food intake (not only speed). Does anybody know whether the calcein is it taken up by the gut cells once it is processed in the gut lumen or whether it is (eventually) expelled by defecation ??
Thanks,
TJ