Drug treatments on NGM plates


Has anybody tried to apply drugs directly on NGM plates and check for effect on worms or are liquid cultures the best way for drug treatments?


I’ve done drug treatments by directly applying 50-100 uL onto the surface of the agar as well as adding it directly to molten agar before pouring plates. I got about the same effect by concentration between the two with drugs I’ve worked with, but I’m sure it can vary. I have to use a relatively high dosage to penetrate the cuticle. If you do apply drug to surface of agar be sure to give it at least 3 hours to diffuse into the agar. Lots of literature out there with this kind of exposure. Off top of my head I can think of drugs like fluoxetine and other SSRI’s having this kind of drug exposure in c elegans for specific protocols.