Drug treatments on NGM plates

Model Organism: Worms Methods & Reagents
1

Hi,

Has anybody tried to apply drugs directly on NGM plates and check for effect on worms or are liquid cultures the best way for drug treatments?

Thanks

2

I’ve done drug treatments by directly applying 50-100 uL onto the surface of the agar as well as adding it directly to molten agar before pouring plates. I got about the same effect by concentration between the two with drugs I’ve worked with, but I’m sure it can vary. I have to use a relatively high dosage to penetrate the cuticle. If you do apply drug to surface of agar be sure to give it at least 3 hours to diffuse into the agar. Lots of literature out there with this kind of exposure. Off top of my head I can think of drugs like fluoxetine and other SSRI’s having this kind of drug exposure in c elegans for specific protocols.