DsRed or mCherry targeted to mitochondria?

Model Organism: Worms Methods & Reagents
1

Dear colleagues

I will very much appreciate to know if anyone has any construct targeting DsRed or mCherry to mitochondria.

Thank you very much in advance
All the very best
Antonio

2

I have a Gateway entry clone that is mito::mCherry, but it hasn’t been published yet.
Are you Gateway-compatible?

If not, there should be a few in Addgene.

Chris

3

If you can’t find one that is ready for use, it should be possible to make one, for example by combining these two previous Wormbase Forum threads.

4

some fire lab vector like pPD96.32 express a mit::gfp, u can change the gfp with mcherry. i think it will work.

5

Dear Chris,
I am a post-doc at the Sternberg lab working on mitochondria. I wonder whether it would be possible to obtain the mito::mCherry entry clone you have. I am looking for way to mark mitochondria in red and this seems ideal. I know it is unpublished but still hoping that you are willing to share it.
Thank you in advance for your help,
Amir