estimating protein concentration

Hi All,
Has anyone here worked with bradford reagent for protein assay. I wished to know how do you exactly determine the concentration of your test sample with a standard curve? Do you take the absorbance/dilution factor into account? It would be really helpful if someone could share the detailed protocol with me.


Ah, the wonder of Google, 1,480,000 pages on Bradford assays and the 4th link describes in simple terms the use of the standard curve so I don’t need to…

Good luck with your calculations.