Dear wormers
Can anyone let me know whether Cytomegalovirus plasmid can be expressed in C. elegans? (by microinjection)
thanx
Dear wormers
Can anyone let me know whether Cytomegalovirus plasmid can be expressed in C. elegans? (by microinjection)
thanx
Hi…
I am also interested in answer…
Can we use CMV promoter to express gene of interest in worms?
Thanks
I’ve never seen any reports using a CMV promoter in worms. It’s been reported that CMV does not work in insect S2 and Kc1 cells (Gene 1997 Apr 1;188(2):183-90) and other reports saying it works it Drosophila cells (Nucleic Acids Res 1987 Mar 11;15(5):2392). Unless you want to do some exploratory work, you might want to stick with a characterized promoter.
If i’m not wrong, I have seen a talk recently from Ursula Schaffer from the Baumeister lab, and she was currently working on making the Tet-On/Tet-Off system in C.elegans.
When she established the system she was using the CMV promoter as a minimal promoter, but apparently it was not working then she had to switch to pes-10 minimal promoter.