Expression of CMV plasmid in C. elegans??

Model Organism: Worms Scientific Discussion
1

Dear wormers

Can anyone let me know whether Cytomegalovirus plasmid can be expressed in C. elegans? (by microinjection)

thanx

2

Hi…

I am also interested in answer…
Can we use CMV promoter to express gene of interest in worms?

Thanks

3

I’ve never seen any reports using a CMV promoter in worms. It’s been reported that CMV does not work in insect S2 and Kc1 cells (Gene 1997 Apr 1;188(2):183-90) and other reports saying it works it Drosophila cells (Nucleic Acids Res 1987 Mar 11;15(5):2392). Unless you want to do some exploratory work, you might want to stick with a characterized promoter.

4

If i’m not wrong, I have seen a talk recently from Ursula Schaffer from the Baumeister lab, and she was currently working on making the Tet-On/Tet-Off system in C.elegans.

When she established the system she was using the CMV promoter as a minimal promoter, but apparently it was not working then she had to switch to pes-10 minimal promoter.