Hello,
I am having what appears to be a problem with the EG6699 strain that we obtained from the CGC. This strain was developed by the Jorgensen lab for single-copy insertions via MosSCI.
The strain is: ttTi5605 II; unc-119(ed3) III; oxEx1578. oxEx1578 is: [eft-3p::GFP + Cbr-unc-119]
I understand that they are supposed to lose the extrachromosomal array at certain frequency, producing uncoordinated animals that do not fluoresce, and that these are what you inject. Rescue of the unc phenotype in progeny indicates successful injection.
The problem we are having is that ALL of our animals have Wild-type movement, and all are fluorescent. This appears to be the case at 20C and at 25C. Is it possible that some integration event has occured? Or, is the heritability of the array very high?
If anyone has experience with this strain, or with similar phenomenon, I would like to hear your input.