FastPrep-24 (MP Biomedicals) Protocol to Grind Up Worms

Model Organism: Worms Methods & Reagents
1

Hello,

Does anyone have a protocol for using the FastPrep-24 to grind up worms for biochemical analysis (such as ATP assays, Western Blots, that sort of thing).

Thanks!

2

We use the Precellys 24, which is I think comparable to the FastPrep-24. We crack them with Zirconium Beads (0.5mm diameter) at 2x6000rpm for 5-10 seconds. We use it for RNA and Protein extraction.

good luck.
Marlon