Filipin staining


If someone has a detailed protocol to stain cholesterol with filipin in our worms, can you please pass me.

Thanks. :slight_smile:

I took the hitherto unprecedented step of typing cholesterol, filipin and elegans into Google…I’m not sure why…it just happened.

Reassuringly, the first entry was this:

read the methods section (Fluorescent staining of free cholesterol, LDs, and lysosomes)…it kind of sets you up to read the second Google entry:

which cites this paper…and BINGO they have a protocol:

I love search engines…they do exactly what you think they might do.

Thank you Sir.

This Filipin and Nile Red, are they same?

not even close…