Filipin staining

Model Organism: Worms Scientific Discussion
1

Hello,

If someone has a detailed protocol to stain cholesterol with filipin in our worms, can you please pass me.

Thanks. :slight_smile:

2

I took the hitherto unprecedented step of typing cholesterol, filipin and elegans into Google…I’m not sure why…it just happened.

Reassuringly, the first entry was this:

http://www.nature.com/srep/2015/150122/srep07930/full/srep07930.html

read the methods section (Fluorescent staining of free cholesterol, LDs, and lysosomes)…it kind of sets you up to read the second Google entry:

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0033962#pone.0033962-Merris1

which cites this paper…and BINGO they have a protocol:

http://www.jlr.org/content/44/1/172.full.pdf+html

I love search engines…they do exactly what you think they might do.

3

Thank you Sir.

This Filipin and Nile Red, are they same?

4

not even close…