Does anyone know where I can find a guide to deciphering the various descriptions of the Fire Lab Vectors (e.g. dpy30Tsa/gfpN/IVSR/gfpS65C/IVSS/IVST/gfpC/let8583’/decoy/)? I’m particularly worried about the “decoy”, bit, which I just noticed.
I attempted to make a translational fusion using the ppD152.79 vector (L5494, http://www.addgene.org/1704/ ), and it looks in frame and correct by sequencing, but I haven’t been able to generate any transgenics by injection (no problem with the empty vector though). This has occurred for 2 different fusion proteins, so I don’t think it’s the actual genes themselves giving me the problem. If you have any information specific to this vector, or can suggest a vector (from personal experience) in the collection for ubiquitous expression of a gene::GFP fusion (preferably with Not, XMA and KPN1 sites for cloning), please let me know.
Edit: Sorry if something like this is covered in previous posts, for some reason the search feature doesn’t seem to be working on this browser.