As a part of migration from the Worm Community Forum, this post was manually created on behalf of the user jac.jacs; when they join the forum here, they can claim this post by replying to this topic.
We are hoping to generate fluorescently tagged non-elegans Caenorhabditis so that we can easily track multiple individuals in a mixed population. We’d love advice and recommendations for genes to tag via CRISPR. We want bright fluorescence, ideally localized to a single region of the worm, for easy counting and tracking. What gene should we target?