Fluorescent tagging via CRISPR

Model Organism: Worms Methods & Reagents
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We are hoping to generate fluorescently tagged non-elegans Caenorhabditis so that we can easily track multiple individuals in a mixed population. We’d love advice and recommendations for genes to tag via CRISPR. We want bright fluorescence, ideally localized to a single region of the worm, for easy counting and tracking. What gene should we target?

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How about using Brainbow, expressed in pharynx. This would help avoid ambiguity when individuals cross paths.

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For this purpose in C. elegans, myo-2 has been used successfully. Its promoter is sufficiently strong that myo-2p::GFP expression can be seen under a GFP dissecting scope even with moderately high white light illumination (see image).