FlyBase has updated the Papers with Technical Advances page (April)

Here’s the new papers:

A genetic screen to uncover mechanisms underlying lipid transfer protein function at membrane contact sites.
Life Sci Alliance
Set of 52 genes were discovered in the paper that interact genetically with rdgB.

dTBI2: A Calibrated, Tunable Device for Administering Traumatic Brain Injury in Drosophila.
Curr Protoc
Method for building a device for administering time- and depth- calibrated closed-head injury to Drosophila.

Mass isolation of staged Drosophila pupal intestines for analysis of protein ubiquitylation.
STAR Protoc
This is a protocol for the mass isolation of staged pupal intestines during Drosophila melanogaster development based on buoyancy in sucrose for biochemical evaluation of protein ubiquitylation. This protocol can be extended to investigate biochemical changes in other tissues.

An automatic system for recognizing fly courtship patterns via an image processing method.
Behav Brain Funct
automatic system for recognizing fly courtship

A live-imaging protocol for tracking intestinal stem cell divisions in the Drosophila melanogaster pupal midgut.
STAR Protoc
ex-vivo live imaging of Drosophila pupal midgut intestianl stem cell divisions.

High-throughput genetic manipulation of multicellular organisms using a machine-vision guided embryonic microinjection robot.
This paper describes an automated robotic microinjection system for Drosophila and zebrafish.

Tissue-specific O-GlcNAcylation profiling identifies substrates in translational machinery in Drosophila mushroom body contributing to olfactory learning.
We have developed a tissue-specific O-GlcNAcylation profiling tool to enrich candidate substrates. Through this tool, we mapped the O-GlcNAc interactome and created an O-GlcNAc atlas for various brain regions of Drosophila (tsOGA, The Drosophila Brain Explore O-GlcANc Database).
Tissue-specific O- GlcNAcylation profiling identifies substrates in translational machinery in Drosophila mushroom body contributing to olfactory learning - PubMed

Hox gene-specific cellular targeting using split intein Trojan exons.
Proc. Natl. Acad. Sci. U.S.A.
Split inteins are incorporated into Trojan-exon (Diao F et al, 2015) to make gene specific drivers.

Two distinct waves of transcriptome and translatome changes drive Drosophila germline stem cell differentiation.
A method to synchronise female germline stem cell differentiation in vivo, generating sufficient material for 'omics or biochemistry experiments at specified stages of differentiation. The study includes RNA-seq and Ribo-seq from this material, at 6 stages of differentiation.

Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila.
STAR Protoc
The use of pseudo-time analysis in single-embryo RNA-sequencing data to determine developmental stage instead of manual embryo staging, thus avoiding human errors. Furthermore, we validated an approach to identify male and female embryos using their transcriptomes.