I’m about to start lifespan assays with FUDR. How do you decide what concentration to use? Does it depent on the strains and needs to be calibrated?
Thanks in advance,
Orit
Never used it I’m afraid, but beware. It has effects on lifespan, morphology and motility and doesn’t affect all worm strains the same, e.g. FUDR increases lifespan of gas-1 mutants 2-fold but has little effect on wild-type.
I’m sure in some cases FUDR can be useful, especially if you have a mutant that bags a lot, just as long as you’re aware of the drawbacks. I’m sure someone else can answer your questions about concentrations
Best of luck with the experiments!
Ben
Aitlhadj, L. and Sturzenbaum, S. R. (2010). The use of FUdR can cause prolonged longevity in mutant nematodes. Mech Ageing Dev. 131, 364-365
Van Raamsdonk, J. M. and Hekimi, S. (2011). FUdR causes a twofold increase in the lifespan of the mitochondrial mutant gas-1. Mech Ageing Dev. 132, 519-521.
Bolanowski, M. A., Russell, R. L. and Jacobson, L. A. (1981). Quantitative measures of aging in the nematode Caenorhabditis elegans. I. Population and longitudinal studies of two behavioral parameters. Mech Ageing Dev. 15, 279-295.
Glenn, C. F., Chow, D. K., David, L., Cooke, C. A., Gami, M. S., Iser, W. B., Hanselman, K. B., Goldberg, I. G. and Wolkow, C. A. (2004). Behavioral deficits during early stages of aging in Caenorhabditis elegans result from locomotory deficits possibly linked to muscle frailty. J Gerontol A Biol Sci Med Sci. 59, 1251-1260
Hi Orit,
Check out the supplementary material in Ref #2 that Ben gave (van Raamsdonk and Hekimi, 2011).
Jeremy did a nice job analyzing the effect of FUdR at a wide range of concentrations (Figure S4).
At concentrations of 1μM FUdR and higher it is not difficult to distinguish parents from progeny on day 3 of adulthood. Thus, a lifespan study can be conducted at 1 μM FUdR if the worms are transferred after 3 days of adulthood and they will not produce any progeny on the second plate.
Have fun!