Hello,
I was looking for publications where aequorin-probes would be used to measure mitochondrial calcium-concentrations in C.elegans. However, I mainly find
papers with fluorescent probes like cameleons (YC3.6). I read that the disadvantages of bioluminescent probes are:
- the low photon emission rate which makes it difficult to obtain subcellular resolution
- the irreversible nature of the luminescence limits the duration of measurements
Has anyone tried to use aequorin probes for mt-Ca?
Is the result really so bad?
Might there be an issue with the cell membrane permeability for coelenterazine?
one might ask " why don´t you just use cameleons?" it´s because I use aequorin-probes for cell experiments and wanted to repeat the
experiments in C.elegans and so almost everything would be set-up to measure mtCa with aequorin-probes.
Many thanks in advance 