genetic mitochondrial Ca-sensor: Aequorin or cameleons?


I was looking for publications where aequorin-probes would be used to measure mitochondrial calcium-concentrations in C.elegans. However, I mainly find
papers with fluorescent probes like cameleons (YC3.6). I read that the disadvantages of bioluminescent probes are:

  1. the low photon emission rate which makes it difficult to obtain subcellular resolution
  2. the irreversible nature of the luminescence limits the duration of measurements

Has anyone tried to use aequorin probes for mt-Ca?
Is the result really so bad?
Might there be an issue with the cell membrane permeability for coelenterazine?

one might ask " why don´t you just use cameleons?" it´s because I use aequorin-probes for cell experiments and wanted to repeat the
experiments in C.elegans and so almost everything would be set-up to measure mtCa with aequorin-probes.

Many thanks in advance :slight_smile: