Hi,
I generated transgenic lines expressing extrachromosomal arrays with 80% transmission ratios and soon need to
generate stable lines using UVcross-link integration method. However, my transgenic lines have too high transmission
ratios. I don’t know why. I tried injecting 10ng/ul, 40ng/ul and 50 ng/ul of DNA of interest and always get lines with super
high transmission ratios and can’t use these lines for integration. How can I get a line with a lower transmission ratio
(~30-50%)?
Thank you in advance!
Foodqueen