Hi, guys, We want to take experiments using a GFP expressing Escherichia coli OP50-GFP or HB101-GFP (C. elegans Genetics Center) to trace
the food in C.elegans, but we don’t know how to get the strains. If someone have these stains, could you mail some to us. We will be greatly appreiated.
I assume you mean bacteria transformed with a plasmid expressing GFP?
You can obtain OP50 carrying a GFP-expressing plasmid from the CGC,
Strain: OP50-GFP
Species: Escherichia coli
Genotype: E. coli.
Description: A strain of OP50 that contains a GFP plasmid (pFVP25.1) that is very fluorescent. Resistant to ampicillin. Originally published in Caenorhabditis elegans is a model host for Salmonella typhimurium. Labrousse A, Chauvet S, Couillault C, Kurz C, Ewbank J. Curr Bio 10(23):1543-45 2000. Biosafety Level: BSL-1.
Mutagen:
Outcrossed: x
Made by: Jonathan Ewbank
Received: 01-19-2001 from Ewbank J, Centre d’Immunologie, Marseille, France
It costs a few Dollars for a starter plate and a bit more to get it sent to you in the post. The necessary details for registering, shipping, payments etc. are well explained on the website.
Thank you, but it will take a long time about two month, we need it for experiments recently
Where could you be then that takes 2 months for a postal delivery (except of course the UK)? The CGC is very reliable and normally it doesn’t take much more than a week if they send it by snail mail.
If it really took 2 months then I doubt the plate would be useable, plus if you found anyone that could send out a similar bacterial culture, it would take the same amount of time to reach you.
You may have to plan/schedule your experiments differently while you wait for the CGC strain.
I am in China, could the CGC mail the strain to us in two weeks?
I would send them an email and ask about posting to China.
email: cgc@umn.edu
They might suggest using some form of courier (FedEx or similar) to speed things up.
Alternatively you could label the bacteria with conjugated FITC or similar.
You could also ask the CGC if they have recently sent OP50-GFP to any labs in China, and if so they could contact those labs and ask if they’d be willing to send you a sample.
I don’t know the CGC policy on confidentiality; I would hope and assume they don’t disclose what they’ve shipped to whom. Still, they might in theory be willing to contact recipients themselves, rather than tell you who those recipients are; also, finding out someone has received OP50-GFP is a lot less of a revelation about results they may not be ready to talk about than is, for example, revealing what particular knockout mutants someone has been requesting.
and you could also send an email to researchers in China who used OP50-GFP in published papers?
Ref. (I didn’t search carefully and they may be incorrect)
http://www.ncbi.nlm.nih.gov/pubmed/21721562
http://www.ncbi.nlm.nih.gov/pubmed/24756229
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0052392