glutathione measurements?

Model Organism: Worms Methods & Reagents
1

Can anyone recommend a good method for measuring total GSH and GSH/GSSG ratios in worms?

Thanks,

2

Perfect world scenario? HPLC (Schulz et al., Schmeisser et al.).

Sigma also has a kit. I’ve never tried it, but my labmate says it’s great.

3

I used LC-MS/MS to measure GSH and GSSH in worms. I performed on API 2000 machine (It’s a triple quadrupole mass), with MRM (multiple reaction mornitoring) mode.