Growing worms for protein extraction

Model Organism: Worms Methods & Reagents
1

So I have read some differing protocols out there for protein extraction, and I am trying to find the best for a neural cytosolic protein (UNC-18).
Some questions I have:

  1. For growing up worms why use egg plates or NA22 in place of added cholesterol and OP50?
  2. Liquid or solid culture, which is better?
  3. Protein disruption, what are the pros and cons of these? Sonication, French Press, homogenizer etc.

Thanks!

April Reedy
Université Claude Bernhard
Lyon, France