So I have read some differing protocols out there for protein extraction, and I am trying to find the best for a neural cytosolic protein (UNC-18).
Some questions I have:
- For growing up worms why use egg plates or NA22 in place of added cholesterol and OP50?
- Liquid or solid culture, which is better?
- Protein disruption, what are the pros and cons of these? Sonication, French Press, homogenizer etc.
Thanks!
April Reedy
Université Claude Bernhard
Lyon, France