Hi,
I would like to cross one of the Spe-9 mutant strains (either hc52 or hc88 allele) into the strain I am currently working with, so that I can shift my experiments to 25C and avoid having to transfer daily or use FUDR for later time points in my experiments. I read some literature on the different alleles of Spe-9, but am still having a hard time deciding which one to use. Please advise! (or suggest a different strain?) Thanks!