Hello everyone!
I need to kill [i] E.coli [/ i] for my trials. For that reason I would like to know the best protocol to effectively kill the e.coli
Thank you very much.
Eva,
If you search on the web, you can find several methods for killing E. coli to feed worms.
Treatment for 30 min at 70C or 10 min at 80C or 30 min with UV will definitely kill the bacteria.
You can test the bacteria by trying to grow them in broth.
Note that there are reports in the literature that lifespan is different for worms grown on live vs. dead. bacteria.
Good luck, Janet