I’ve performed Liquid killing assay (in M9 Buffer) for C. elegans by comparing N2 with mutants for specific bacterial infection condition (Contains both ‘control’ and ‘Infected’). I’ve read articles and GraphPad prism for how to enter the raw data for making the survival graph. My confusion is that none in those guidelines have discussed about liquid killing assay format but had given with solid only. Because there is two column ‘Dead’ and ‘Censored’ which is confusing me. Can anyone simplify how to enter the data in ‘X’ and ‘Y’ columns for “LIQUID”. It’d be better if someone send a model data sheet for ‘Liquid’. I really appreciate for your help. Thank you.
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