Dear all,

i was trying to calculate the TD50 for my survival analysis but i am messed up somehow the way to define it in GraphPad…

Most literature illustrate the equation as follows:

Y = Bottom + [Top − Bottom]/[1 + 10(log EC50 − X)(Hill slope)],

where X is the logarithm of days, Y is the average of dead worms (3), and EC50 is the midpoint of the curve.

I have attached my GrapPad analysis here and hope if someone may help me out. Appreciate that!

Here is my dropbox link to the graphpad file

https://www.dropbox.com/s/tencnx4wfdxfr1y/TD50.pzf

cheers