i was trying to calculate the TD50 for my survival analysis but i am messed up somehow the way to define it in GraphPad…
Most literature illustrate the equation as follows:
Y = Bottom + [Top − Bottom]/[1 + 10(log EC50 − X)(Hill slope)],
where X is the logarithm of days, Y is the average of dead worms (3), and EC50 is the midpoint of the curve.
I have attached my GrapPad analysis here and hope if someone may help me out. Appreciate that!
Here is my dropbox link to the graphpad file