How to insert TD50 equation in GraphPad

Model Organism: Worms Methods & Reagents
1

Dear all,

i was trying to calculate the TD50 for my survival analysis but i am messed up somehow the way to define it in GraphPad…

Most literature illustrate the equation as follows:
Y = Bottom + [Top − Bottom]/[1 + 10(log EC50 − X)(Hill slope)],
where X is the logarithm of days, Y is the average of dead worms (3), and EC50 is the midpoint of the curve.

I have attached my GrapPad analysis here and hope if someone may help me out. Appreciate that!
Here is my dropbox link to the graphpad file
https://www.dropbox.com/s/tencnx4wfdxfr1y/TD50.pzf

cheers

2

Hi Tyrael,

Actually, GraphPad has that equation built in :smiley: It’s called Sigmoidal dose-response (variable slope).

Insert → New Analysis → Non-linear regression → Sigmoidal dose-response (variable slope)

I cloned the equation and entered the new/edit mode of the equation editor so you can see it:

http://i.imgur.com/pLHokbl.jpg

3

Oh my god! It’s just within a click!
Snug, you have just saved my day! Thank you so much!!! :smiley: ;D