Is there a paralyzed mutant that stays still enough for confocal imaging? If so, why does everyone prefer to use sodium azide or levamisole?
It’s a pain to make a double mutant whenever you want to image.
We use a few µl of 10 mM muscimol (in water) on the 100µl pad instead of levamisole. It takes a bit longer, but the animals stay much more still during imaging. The worms also relax and stretch out which makes the worms look better.
You could also use the polystyrene beads developed by Chris Feng-Yen. Many labs swear by it: