Just wondering whether there is any evidence that MosSci HR-directed repair is more effective in NHEJ mutants, eg. cku-70/80 worms?
To my knowledge, no-one in the Jorgensen lab has tried the NHEJ mutants, even though we discussed it at one point. We don’t have a very good estimate of what proportion of the repair proceeds down the different pathways, but I think the Bessereau lab has looked a little more carefully. My guess is that the NHEJ mutants would help for making larger deletions.
- christian