Ingredients for making NGM plates

Model Organism: Worms Methods & Reagents
1

Hello,

Can somebody please tell me in short the function of the following ingredients which are used for making NGM plates?

CaCl2, MgS04, Cholesterol, K2HPO4, KH2PO4, NaCl, Peptone.

Any input will be highly appreciated.

Thanks.

2

Hi Sanjib,

you may refer to this paper "Virulence of Leucobacter chromiireducens subsp. solipictus to Caenorhabditis elegans: Characterization of a Novel Host-Pathogen Interaction " which tell the function of cholesterol

“… Addition of 0.5% cholesterol to media used for nematode growth-related studies fulfills the requirement of exogenous sterols for C. elegans development…”

cheers,
edward