I am looking for an integrated strain with a pan-neuronal nuclear GFP (or mCherry, XFP etc.) marker. I have found gaIs234[jkk-1p::HIS-24::mCherry + unc-119(+)] but no other strains in CGC. Any suggestion would be very welcome.
[Edit: Whoops. I didn’t initially catch nuclear :-[ ]
Hi. I downloaded the latest CGC file to my computer from here: http://www.cbs.umn.edu/CGC/strains/gophstrnt.txt. Then I did a search of the local file for “pan-” and “panneuronal” and this is what came up:
Strain: OH439
Description: Integrated Ex[unc-33::GFP; unc-4(+)] in unc-4(-) background. Pan-neural expression. No LG info for otIs118. May have unc-4(e120) in background still.
Strain: OH441
Description: Integrated Ex[unc-119::GFP] in N2 background. No injection marker. Pan-neural expression.
Strain: OH7547
Description: otIs199 [cat-2::GFP + rgef-1(F25B3.3)::dsRed + rol-6(su1006)]. Rollers. GFP expressed in dopaminergic neurons and dsRed expressed panneuronally.
Yes, there are many strains with a pan-neuronal GFP marker. But pan-neuronal nuclear GFP markers are few.
Strains with nuclear GFP markers expressed in many neurons would help me.