integrated strain with a pan-neuronal nuclear GFP

Model Organism: Worms Methods & Reagents
1

Hi everyone,

I am looking for an integrated strain with a pan-neuronal nuclear GFP (or mCherry, XFP etc.) marker. I have found gaIs234[jkk-1p::HIS-24::mCherry + unc-119(+)] but no other strains in CGC. Any suggestion would be very welcome.

Thank you in advance for your help.

2

[Edit: Whoops. I didn’t initially catch nuclear :-[ ]

Hi. I downloaded the latest CGC file to my computer from here: http://www.cbs.umn.edu/CGC/strains/gophstrnt.txt. Then I did a search of the local file for “pan-” and “panneuronal” and this is what came up:

Strain: AM44
Description: rmIs190 [F25B3.3p::Q67::CFP]. Pan-neuronal CFP expression.

Strain: AM49
Description: rmIs172 [F25B3.3p::Q19::CFP]. Pan-neuronal CFP expression.

Strain: AM101
Description: rmIs110 [F25B3.3p::Q40::YFP]. Pan-neuronal YFP expression.

Strain: NW1229
Description: Integrated Ex[F25B3.3::GFP; dpy-20(+)] in dpy-20(-) background. Pan-neural expression. No LG info for evIs111.

Strain: TU3310
Description: uIs59 [unc-119p::YFP]. Pan-neuronal YFP expression.

Strain: OH438
Description: otIs117[unc-4(+) + unc-33p::GFP]. Panneuronal GFP.

Strain: OH439
Description: Integrated Ex[unc-33::GFP; unc-4(+)] in unc-4(-) background. Pan-neural expression. No LG info for otIs118. May have unc-4(e120) in background still.

Strain: OH441
Description: Integrated Ex[unc-119::GFP] in N2 background. No injection marker. Pan-neural expression.

Strain: OH7547
Description: otIs199 [cat-2::GFP + rgef-1(F25B3.3)::dsRed + rol-6(su1006)]. Rollers. GFP expressed in dopaminergic neurons and dsRed expressed panneuronally.

3

Thank you very much for the reply :slight_smile:

Yes, there are many strains with a pan-neuronal GFP marker. But pan-neuronal nuclear GFP markers are few.
Strains with nuclear GFP markers expressed in many neurons would help me.

4

It it beginning to look like it may be faster, unfortunately, to make the strain…if you have the means to do so.