integrated strain with a pan-neuronal nuclear GFP

Hi everyone,

I am looking for an integrated strain with a pan-neuronal nuclear GFP (or mCherry, XFP etc.) marker. I have found gaIs234[jkk-1p::HIS-24::mCherry + unc-119(+)] but no other strains in CGC. Any suggestion would be very welcome.

Thank you in advance for your help.

[Edit: Whoops. I didn’t initially catch nuclear :-[ ]

Hi. I downloaded the latest CGC file to my computer from here: http://www.cbs.umn.edu/CGC/strains/gophstrnt.txt. Then I did a search of the local file for “pan-” and “panneuronal” and this is what came up:

Strain: AM44
Description: rmIs190 [F25B3.3p::Q67::CFP]. Pan-neuronal CFP expression.

Strain: AM49
Description: rmIs172 [F25B3.3p::Q19::CFP]. Pan-neuronal CFP expression.

Strain: AM101
Description: rmIs110 [F25B3.3p::Q40::YFP]. Pan-neuronal YFP expression.

Strain: NW1229
Description: Integrated Ex[F25B3.3::GFP; dpy-20(+)] in dpy-20(-) background. Pan-neural expression. No LG info for evIs111.

Strain: TU3310
Description: uIs59 [unc-119p::YFP]. Pan-neuronal YFP expression.

Strain: OH438
Description: otIs117[unc-4(+) + unc-33p::GFP]. Panneuronal GFP.

Strain: OH439
Description: Integrated Ex[unc-33::GFP; unc-4(+)] in unc-4(-) background. Pan-neural expression. No LG info for otIs118. May have unc-4(e120) in background still.

Strain: OH441
Description: Integrated Ex[unc-119::GFP] in N2 background. No injection marker. Pan-neural expression.

Strain: OH7547
Description: otIs199 [cat-2::GFP + rgef-1(F25B3.3)::dsRed + rol-6(su1006)]. Rollers. GFP expressed in dopaminergic neurons and dsRed expressed panneuronally.

Thank you very much for the reply :slight_smile:

Yes, there are many strains with a pan-neuronal GFP marker. But pan-neuronal nuclear GFP markers are few.
Strains with nuclear GFP markers expressed in many neurons would help me.

It it beginning to look like it may be faster, unfortunately, to make the strain…if you have the means to do so.