Hi All,
Has anyone here designed or made constructs for isoform specific knockdown of a gene by RNAi? Isoforms for my gene of interest differ in the first exon. The exon lengths are betwwen 80-150 bp for the isoforms. Is this length sufficient to achieve isoform specific knockdown since most of the RNAi vectors I have studied or found contain at least 500bp of the coding sequence to knockdown gene of interest.
thanks