Isolation of Mitochondria proteins -regarding

Model Organism: Worms Scientific Discussion
1

Whether there is any kit for isolation of C. elegans Mitochondria proteome.

Thanking you for providing me a link or copy of that protocol (dilawerjk@gmail.com)

Yours obediently

Dilawer Mir
Kashmir

2

You might want to contact the authors of this paper, they seem to have a protocol:

Proteomic analysis of mitochondria from Caenorhabditis elegans.
Li J, Cai T, Wu P, Cui Z, Chen X, Hou J, Xie Z, Xue P, Shi L, Liu P, Yates JR 3rd, Yang F.
There’s also this methods paper:
Isolation and functional analysis of mitochondria from the nematode Caenorhabditis elegans.
Grad LI, Sayles LC, Lemire BD.

3

Hi Dilawer,

Attached the mentioned papers for you in an e-mail. Check that

Also, If you can get enough quantity of Mitochondria you can use the conventional proteomics approach. Have you quantified the concentration using RIPA buffer method?

Check this link may be useful if you find a local supplier

http://www.inventbiotech.com/store/index.php?main_page=product_info&cPath=2&products_id=180 [/u]

Thanks
Durai