Whether their is any Percoll based ultracentrifuge density gradient protocol for isolation of C elegans whole Mitochondrial proteome,
Thanking you to provide me link or copy of that protocol.
Yours obediently
Dilawer Mir
Kashmir
Whether their is any Percoll based ultracentrifuge density gradient protocol for isolation of C elegans whole Mitochondrial proteome,
Thanking you to provide me link or copy of that protocol.
Yours obediently
Dilawer Mir
Kashmir
Hi,
take a look at this paper, hopefully you have access to it through your institution;
(why do they make these links so long? ???)
They use Nycodenz (presumably to avoid some of the issues with Percoll), but the description is pretty comprehensive.
Steve