The FlyBase Papers with Technical Advances page has updated with papers submitted in July.
If you’d like to be featured in an upcoming update, please flag your paper with a new resource, technique, or reagent when you Fast-Track Your Paper.
NAD[+] supply and redox state limit developmental speed in the Drosophila eye. (EMBO J.)
new genetically encoded-metabolic fluorescent biosensors for ATP and NADH/NAD+ ratio (transgenes)
https://pubmed.ncbi.nlm.nih.gov/42129544
Chemical programming of kinase inhibitors in a modular chemputer-based system. (Commun. Biol.)
Digital chemistry is used in phenotype-driven QSAR
https://pubmed.ncbi.nlm.nih.gov/41896327
Super-resolution imaging of cell death in Drosophila tissues via expansion and pan-expansion microscopy. (Methods Cell Biol.)
We describe two ExM-based protocols for Drosophila tissues: ExM (~4x expansion) and pan-ExM (~12-16x). ExM enables super-resolution imaging of immunofluorescent labels, while pan-ExM reveals ultrastructural features but offers limited specific protein detection.
https://pubmed.ncbi.nlm.nih.gov/42276709
Whole brain fluorescence imaging in Drosophila reveals spreading depolarization and its initiation, propagation, and resilience dynamics. (iScience)
High-throughput, whole-brain fluorescence imaging assays in Drosophila melanogaster ideal for observing and mechanistically understanding spreading depolarization.
https://pubmed.ncbi.nlm.nih.gov/42375524
New cell lines derived from larvae of the neotropical fruit fly Drosophila willistoni persistently infected with Wolbachia. (Fly)
Three new cell lines derived from larvae of Drosophila willistoni, available as sublines both naturally-infected with Wolbachia strain wWil and cured of Wolbachia infection by tetracycline treatment.
https://pubmed.ncbi.nlm.nih.gov/42332854
Energy-efficient information processing and eligibility-trace plasticity in the Drosophila optic lobe connectome. (Sci. Rep.)
Computational framework and open-source resource for connectome preprocessing, structural MDL analysis, neural dynamics, energy-information estimation, and eligibility-trace plasticity in the Drosophila optic lobe.
https://pubmed.ncbi.nlm.nih.gov/42156870
Proximal labeling of the Golgi secretome reveals fat body-derived humoral factors in Drosophila disc regeneration. (J. Biol. Chem.)
UASz-Mgat2-V5-TurboID, a Golgi-targeted TurboID reporter that enables proximity labeling of Golgi-transiting secreted proteins and membrane proteins in Drosophila tissues.
https://pubmed.ncbi.nlm.nih.gov/42208902
Autoinhibitory feedback preserves intestinal stem cell maintenance and fate commitment. (EMBO J.)
This study provides a comprehensive single cell RNA-seq atlast of the midgut under wild type condition and under progenitor-specific Notch sgRNA condition. This study provides a protein-DNA interactions map using the NanoDam approach for Cph.
https://pubmed.ncbi.nlm.nih.gov/42162240
Somatic cells compartmentalise their carbohydrate metabolism to sustain germ cell survival. (EMBO J.)
Ubi-CanlonicSF (BDSC stock numbers 607049-607050) stocks were generated. These are the same CanlonicSF construct as reported by Aburto et al (FBrf0255149), but with a Ubi promoter for Gal4-independent expression.
https://pubmed.ncbi.nlm.nih.gov/42243520
Light- and temperature-sensitive seizures are regulated by spatially distinct cortex glial populations in the central nervous system. (Proc. Natl. Acad. Sci. U.S.A.)
Cortex Glial subtype specific split-Gal4 and Gal80 driver lines were generated. These include R54D10-Gal4.DBD, R54D10-nlsLexA.p65AD, R54D10- Gal80, R9F07-Gal4.DBD, R9F07-Gal80. We have also generated UAS-Cpes-HA, and UAS-Zyd-HA transgenic lines.
https://pubmed.ncbi.nlm.nih.gov/42479834
Development of an in vivo, screenable, split-luciferase based model of huntingtin multimerization. (iScience)
Developed an in vivo, screenable split-luciferase model of huntingtin multimerization in Drosophila