The FlyBase Papers with Technical Advances page has updated with papers submitted in June
If you’d like to be featured in an upcoming update, please flag your paper with a new resource, technique, or reagent when you Fast-Track Your Paper.
Single-molecule mitochondrial DNA imaging reveals heteroplasmy dynamics shaped by developmental bottlenecks and selection in vivo. (Dev. Cell)
Mitochondrial DNA single-molecule fluorescence in situ hybridisation (mtDNA-smFISH) for in situ visualisation of mtDNA molecules and sequence-specific detection of heteroplasmic variants
https://pubmed.ncbi.nlm.nih.gov/42127801
The mRNA architecture of the translation termination site primes programmed stop codon readthrough events in Drosophila. (RNA Biol.)
A configurable Python script that parses RNAfold dot-bracket notation outputs and annotates the presence of RNA hairpin structures.
https://pubmed.ncbi.nlm.nih.gov/42089732
Investigating the Neural Control of Social Behavior in Drosophila melanogaster Using a Low-Cost Optogenetics System. (J. Undergrad. Neurosci. Educ.)
This paper describes a low-cost platform for performing optogenetic experiments in Drosophila and its implementation in an undergraduate neuroscience laboratory activity.
https://pubmed.ncbi.nlm.nih.gov/42065110
Complementary volume electron microscopy-based approaches reveal ultrastructural changes in germline intercellular bridges. (J. Cell Sci.)
Volume electron microscopy dataset of st4 and germarium ovarian chamber
https://pubmed.ncbi.nlm.nih.gov/42021614
Combining ultrastructure expansion microscopy with immunofluorescence and Oligopaint DNA FISH. (Chromosome Res.)
Ultrastructure Expansion Microscopy combined with immunofluorescence and Oligopaint DNA FISH expands the experimental possibilities for high-resolution studies of chromosomal structures and nuclear organization.
https://pubmed.ncbi.nlm.nih.gov/42162320
Galactose alters early-life development and exerts sex-specific nutritional programming effects on lifespan in Drosophila melanogaster. (Front Nutr)
Oxygen consumption as a proxy for energy expenditure by pupae was measured using a differential O2 analyzer system (Qubit Systems, Kingston, Canada).
https://pubmed.ncbi.nlm.nih.gov/42146084
Light-inducible FLPase reconstitution enables temporal control of gene expression in Drosophila melanogaster. (Cell Rep Methods)
Method to activate gene expression with light, which reconstitutes a split Flp that then removes stop-cassette
https://pubmed.ncbi.nlm.nih.gov/41999763
Drosophila pan-glial inducible Gal4 line alters baseline sleep. (MicroPubl Biol)
Method for building a device for administering time- and depth- calibrated closed-head injury to Drosophila.
https://pubmed.ncbi.nlm.nih.gov/42222857
Drosophila FMRFa neuropeptide signaling modulates systemic glycogen metabolism and fitness in a diet-dependent manner. (Front. Endocrinol.)
In vivo measurement of intracellular glucose levels of muscles and fat body using the FRET-based glucose sensor
https://pubmed.ncbi.nlm.nih.gov/42205263
Genetic analyses enabled by the fourth chromosome resource project reveal unexpected mutant phenotypes and suggest new disease models. (G3 (Bethesda))
Fourth Chromosome Resource Project
https://pubmed.ncbi.nlm.nih.gov/41902692
Tuning mitotic recombination with patterned DNA nicks for precision mosaic analysis. (Proc. Natl. Acad. Sci. U.S.A.)
We establish Cas9 nickases (D10A, H840A) as safer alternatives for somatic mosaic analysis in Drosophila. We define how gRNA nicking patterns tune clone frequency and provide a toolkit (Gateway, HACK donors, LGSR reporter) to generate tissue-specific nickases.
https://pubmed.ncbi.nlm.nih.gov/42201952
Effete and Cullin 4 affect nuclear organization of the gypsy chromatin insulator. (BMC Biol.)
Our ChIP-seq data showed that Cul4, a conserved cullin family protein, exhibits widespread genomic occupancy, strongly co-localizes with CP190, and is predominantly enriched at promoter regions.
https://pubmed.ncbi.nlm.nih.gov/41998702
Spatial, temporal and Notch determination of terminal selector expression controls neuronal cell fate in the Drosophila optic lobe. (Nat. Neurosci.)
Spatial origin of optic lobe neuronal types Correlation between spatial/temporal/Notch origin of optic lobe neuronal types and terminal selector expression
https://pubmed.ncbi.nlm.nih.gov/41992006
Endocytome profiling uncovers cell-surface protein dynamics underlying neuronal connectivity. (Neuron)
Generated transgenes for endocytome profiling: a systematic, cell-type-specific approach for mapping cell-surface protein dynamics (from the plasma membrane to endosomes) in situ.
https://pubmed.ncbi.nlm.nih.gov/41825443
BNIP3-Dependent Mitophagy Non-Autonomously Regulates Systemic Aging via NF-κB Suppression in Drosophila. (Aging Cell)
generate a transgenic fly line mito-SRAI to monitor mitophagy in vivo
https://pubmed.ncbi.nlm.nih.gov/42128879
Sexual identity of Akh neurons shapes Nucb1-dependent metabolic and reproductive plasticity. (iScience)
In this paper, the Leung lab developed a new antibody that detects the N-terminus (residues 25-44) of the protein of interest, Nucleobindin 1 (Nucb1, Uniprot ID: Q9VVK7).
https://pubmed.ncbi.nlm.nih.gov/42291196
Two-photon characterisation of long-Stokes-shift dye ATTO 490LS for single-laser multicolour imaging. (MicroPubl Biol)
Tested ATTO 490 LS in Drosophila for use as a dye.
https://pubmed.ncbi.nlm.nih.gov/42257177
In situ architecture of developmentally programmed mitophagy reveals ER-phagophore membrane continuity. (Autophagy)
This study utilized a combination of different approaches of cryo-ET, cryo-FIB milling, and volume electron microscopy to unveil in situ architecture of forming mitophagosomes in the developing Drosophila intestine.
https://pubmed.ncbi.nlm.nih.gov/41949493
julius seizure acts within teashirt -expressing neurons to protect Drosophila melanogaster from bang-sensitivity. (MicroPubl Biol)
P{y+t7.7 w+mC=GMR90B09-GAL4}attP2, a jus-GAL4 construct that uses the jus promoter to drive GAL4 (BDSC 607421)
https://pubmed.ncbi.nlm.nih.gov/42328358
Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking. (Nat. Commun.)
Developed a Drosophila tissue-secretome resource integrating TurboID-ER proximity labeling, larval hemolymph proteomics, whole-larva snRNA-seq, and CRISPR knock-in strains to map tissue origins and trafficking of circulating proteins.
https://pubmed.ncbi.nlm.nih.gov/42009678
Oligo(dT) Fluorescence In Situ Hybridization to Visualize the Poly(A) mRNAs in the Internal Tissues of Drosophila. (Bio Protoc.)
This technique helps to visualize the distribution of total polyadenylated mRNAs in the cells.