Hello,
Does anybody here have experience in detecting lipase activity in the nematode? Neutral lipase assay and acidic lipase activity detection are different. So the protein preparation processes are also various. I referred to related literature but still cannot get information in detail. Would anybody here show me protocols for protein extraction to prepare for neutral and acidic lipases assay? I would appreciate it.
By the way, I consider using Lipase Assay Kit(QuantiChrom Kit, BioAssay Systems, e.g.) and fluorogenic substrate (4-Methylumbelliferyl palmitate) for neutral and acid lipase detection, respectively.
Armstrong