Does anyone have any experience using either trehalose and betaine, or inert macromolecules to get longer reads with RT-PCR? I would really like full length cDNAs of an alternatively spiced gene.
thanks,
jason
Speiss, A., and Ivell, R. 2002. A highly efficient method for Long-Chain cDNA synthesis using Trehalose and betaine. Analyticial Biochemistry
Lareu, R., Harve, K., and Raghunath, M. 2007. Emulating a crowded intracellular environment in vitro dramatically improves RT-PCR performance. Biochem and Biophysics R. C.