FlyBase has updated the Papers with Technical Advances page FlyBase:Papers with technical advances - FlyBase Wiki!
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Single cell sequencing of pupal to adult midgut development. Provides information about cell types and genes expressed in these as the gut undergoes final adult maturation. The emergence of circadian timekeeping in the intestine. Nat. Commun. PMID:38413599 FBrf0258833
A transgene encoding a membrane-tethered form of Noktochor, a protein that is normally secreted from astrocytes and neurons to regulate sleep. Drosophila noktochor regulates night sleep via a local mushroom body circuit. iScience PMID:38380256 FBrf0258837
A collection of cell-type-specific stable-split GAL4 reagents for courtship song neurons. Fly Light Split-GAL4 Collection Nested neural circuits generate distinct acoustic signals during Drosophila courtship. Curr. Biol. PMID:38295797 FBrf0258823
A new drug stabilizable GAL80ds variant was created by fusing it to a low-background DHFR-22-DD. A drug stabilizable GAL80[ds] for conditional control of gene expression via GAL4-UAS and CRISPR-Cas9 systems in Drosophila. Sci. Rep. PMID:38467687 FBrf0259011
Transformer female-specific introns from three speices (D. melanogaster, D. suzukii, and C. capitata) were tested to show female-specificity at different developmental stages in D. melanogaster. This can be used for female-specific expression study. A Fluorescent Sex-Sorting Technique for Insects with the Demonstration in Drosophila melanogaster. GEN Biotechnol PMID:38415050 FBrf0258926
We generated a tool kit to express each Zika virus protein in Drosophila. A Zika virus protein expression screen in Drosophila to investigate targeted host pathways during development. Dis. Model Mech. PMID:38214058 FBrf0258902
A protocol for studying the neural circuit of female receptivity in Drosophila. A protocol for measuring the sexual receptivity of female Drosophila. STAR Protoc PMID:37703181 FBrf0259009
We have developed a glyoxal-based fast fixation method for IF, FISH and FISH-IF in Drosophila embryos. We also show that some antibodies like Bic-D, Dynein Heavy Chain and a-Actinin that failed to stain in formaldehyde fixed embryos, were stained beautifully with glyoxal fixation Glyoxal-based fixation of Drosophila embryos for immunofluorescence staining and RNA in situ hybridization. STAR Protoc PMID:37405926 FBrf0258995
New Ca2+ imaging probes targets to subcellular compartments. Ca[2+] imaging of synaptic compartments using subcellularly targeted GCaMP8f in Drosophila. STAR Protoc PMID:38198278 FBrf0259069