The FlyBase Papers with Technical Advances page has updated with papers submitted in February. https://wiki.flybase.org/wiki/FlyBase:Papers_with_technical_advances#Mar_2026
If you’d like to be featured in an upcoming update, please flag your paper with a new resource, technique, or reagent when you Fast-Track Your Paper.
Heterogeneous NF-κB activation and enhancer features shape transcription in Drosophila immunity. (Biophys. J.)
Method for imaging live transcription in Drosophila cells using the RhoBAST/SpyRHOsystem
https://pubmed.ncbi.nlm.nih.gov/41520170
Single-cell transcriptomics of the Drosophila ring gland identifies the SoxN-Vvl complex as a key regulator of juvenile hormone biosynthesis. (Proc. Natl. Acad. Sci. U.S.A.)
JH biosensor fly strain
https://pubmed.ncbi.nlm.nih.gov/41650236
A Complex Network-Based Approach for Detecting and Characterizing Power Neurons in Drosophila. (Neuroinformatics)
We apply a complex network-based approach to the complete connectomes of Drosophila in both larval and adult stages. We define the concept of power neurons and how to detect them. We analyze power neurons from both a theoretical and experimental point of view.
https://pubmed.ncbi.nlm.nih.gov/41686314
Optimal neuromuscular performance requires motor neuron phosphagen kinases. (J. Physiol.)
Creation of ArgK1 CRISPR reagents and several reporter transgenes (UAS-Pyronic, UAS-LiLac and UAS-Ateam) along with a novel apparatus for running optogenetic motor neuron performance assays (fully described in PMID 41407019).
https://pubmed.ncbi.nlm.nih.gov/41273355
Resolving synaptic events using subsynaptically targeted GCaMP8 variants. (eLife)
New Ca2+ imaging probes targets to subcellular compartments.
https://pubmed.ncbi.nlm.nih.gov/41769864
Low Angle Ring Illumination Stereomicroscopy (LARIS) Method for High-Contrast Imaging of Drosophila Compound Eyes. (Bio Protoc.)
The LARIS method enables the capture of high-contrast images of the Drosophila compound eye.
https://pubmed.ncbi.nlm.nih.gov/41675996
An RNAi-based platform for spatiotemporal control of histone gene expression during animal development. (Epigenetics Chromatin)
replication-dependent histone gene replacement via RNAi
https://pubmed.ncbi.nlm.nih.gov/41772691
Acrosomal protein NEP4 is required for sperm retention in, but not entry into, the female seminal receptacle in Drosophila melanogaster. (Genetics)
EGFP knock-in allele
https://pubmed.ncbi.nlm.nih.gov/41401818
Three-dimensional genome reorganization foreshadows zygotic genome activation in Drosophila. (Nat. Genet.)
The paper describes Pico-C, a low-input Micro-C approach that enables high-resolution, temporally resolved three-dimensional genome mapping during early Drosophila embryogenesis.
https://pubmed.ncbi.nlm.nih.gov/41735587
A genome-wide MAGIC kit for recombinase-independent mosaic analysis in Drosophila. (eLife)
We developed a complete genome-wide gRNA-marker kit with optimized gRNA and marker designs to enable recombinase-independent mosaic analysis by gRNA-induced crossing-over (MAGIC) across all Drosophila chromosomes, including previously inaccessible centromere-proximal genes.
https://pubmed.ncbi.nlm.nih.gov/41811192
Gene expression cartography of a developing neuronal structure. (Dev. Biol.)
We provide a spatial atlas of gene expression in the developing Drosophila optic lobe, reconstructed from single-cell data using Novosparc. This resource enables exploration of spatial cell states and gene expression patterns across the tissue.