May updates to the FlyBase Papers with Technical Advances page

Papers submitted in May are here FlyBase:Papers with technical advances - FlyBase Wiki

Dynamics of two distinct memory interactions during water seeking in Drosophila. (Proc. Natl. Acad. Sci. U.S.A.)

Establishing a novel humidity memory analysis method in Drosophila.

Characterization of transcriptomics during aging and genes required for lifespan in Drosophila intestine. (Sci. Rep.)

RNA-SEQ of female adult guts at different age, including day-3, -15, -30, -40, and -50; lifespan analysis by esg-Gal4 driven expression of RNAi to knockdown genes expression in intestinal stem cells and enteroblasts.

Comparative single-cell transcriptomic atlases of drosophilid brains suggest glial evolution during ecological adaptation. (PLoS Biol.)

Comparative whole central brain single-nucleus RNA-seq atlases of D. melanogaster, D. simulans and D. sechellia

Quantitative and site-specific chemoproteomic profiling of O-GlcNAcylation in Drosophila. (Bioorg. Med. Chem.)

We perform quantitative and site-specific profiling of O-GlcNAcylation in Drosophila by employing a chemoenzymatic labeling strategy. A total of 2,196 unambiguous O-GlcNAcylation sites and 1,308 O-GlcNAcylated proteins are identified.

PDZD8 promotes autophagy at ER-lysosome membrane contact sites to regulate activity-dependent synaptic growth. (Cell Rep.)

In this manuscript, we have used CRISPR to generate knockouts and endogenously tagged lines for PDZD8. We have also generated multiple tagged PDZD8 transgenic lines.

Machine Learning Scoring Reveals Increased Frequency of Falls Proximal to Death in Drosophila melanogaster. (J. Gerontol. A Biol. Sci. Med. Sci.)

Machine learning scoring of fly falls

The underappreciated, underrecognized problem of fourth chromosome trisomy in Drosophila melanogaster stocks and a simple, general method for building diplo-4 stocks from triplo-4 stocks. (MicroPubl Biol)

Use of the Minute mutation RpS3A[57g] to establish stocks disomic for chromosome 4 from stocks trisomic for chromosome 4.

ReLo is a simple and rapid colocalization assay to identify and characterize direct protein-protein interactions. (Nat. Commun.)

New cell-based method for studying direct protein-protein interactions, designed to accommodate structurally complex proteins (i.e., large proteins with a high degree of disorder).