Measuring ROS in C. elegans using DCFDA

Dear all,

Just wanted to know, anyone tried in measuring ROS in C. elegans using DCDFA?
Because I want to do this experiment, but I find lot of protocols where they sonicate the worms and do in micro-well plates.
But I dont want to this way, I want to do using whole worms.

So if anyone have this protocol, regarding this can you please post.

Thanks a lot.

Hi there

We have recently published the method in the following paper.

The characterization of the Caenorhabditis elegans mitochondrial thioredoxin system uncovers an unexpected protective role of thioredoxin reductase 2 in β-amyloid peptide toxicity.
Cacho-Valadez B, Muñoz-Lobato F, Pedrajas JR, Cabello J, Fierro-González JC, Navas P, Swoboda P, Link CD, Miranda-Vizuete A.
Antioxid Redox Signal. 2012 Jun 15;16(12):1384-400.

Check the supplementary materials and methods.
Should you need further information, please contact me by email and I can put you in touch with the student who optimized the protocol who is now a postdoc somewhere else.

Hope it helps