membrane integrated protein fused with GFP in embryos

Model Organism: Worms Scientific Discussion
1

Does anyone know strains carrying an integration of any membrane-integrated protein fused with GFP that is expressed in 1 cell-embryos?

2

There is a pie-1:SNB-1::GFP used in Green et al. 2011 and Shi et al. 2010. Table S7 of Green et al. shows that this is integrated. There is also RT688, pie-1:CAV-1::GFP, although the GFP disappears quickly after fertilization.

3

Tnanks you :slight_smile:

4

If CAV-1::GFP is useful to you and not too transient after fertilization, there are a few other integral membrane proteins that exist as pie-1 driven gfp strains: chs-1, egg-1, and egg-2.