Has anyone succeeded transfection of c elegans by micro-injecting gonads under Phase contrast without DIC? Is this impossible or just hard to carry out? I concern about this as i do not have DIC optics, we are planning to set up a lab will take long time to acquire DIC. Thanks
Dear Kanishka
Having tried Hoffman contrast and DIC I found DIC much better. I doubt phase contrast alone will be enough. You can also have a look at that previous question: http://forums.wormbase.org/index.php?topic=1006.msg1945;topicseen#msg1945
Good luck
Greg