mRNA injection into worm gonad..?

Model Organism: Worms Methods & Reagents
1

Has anyone injected mRNAs (in vitro transcribed) into worms to produce transient generation of proteins in the gonad? We’re trying to do this for series of gfp- & NLS-tagged proteins but really need a +ve control to compare these against.

2

There are at least a couple of examples of expression of injected mRNAs (capped and polyadenylated in vitro) in the literature:

Evans et al. (1994) Cell 77(2):183-94. PMID: 8168128

Marin and Evans (2003) Development 130, 2623-2632 PMID: 12736207

Abby

3

Thanks Abby, I am looking to see if someone had expressed GFP mRNA rather than Lac Z!