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We are planning to use CRISPR to integrate GFP into myo-2 in C. briggsae. Has anyone successfully tagged myo-2 with CRISPR in Caenorhabditis? Is there a preferred plasmid that will make healthy, bright worms?

Any tips or tricks would be appreciated! Thanks.

Textpresso “myo-2 CRISPR” =>

Early Developmental Exposure to dsRNA Is Critical for Initiating Efficient Nuclear RNAi in C . elegans .
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SECTION: materials. Single-Copy myo-2 : : GFP Transgene This single-copy insertion myo-2 : : GFP transgene ( Norris et al . , 2015 ) was generated by CRISPR / Cas9 genome editing and is inserted at , and disrupts the function of , F32B4 . 4 , which encodes a putative RNA-binding protein

Efficient Genome Editing in Caenorhabditis elegans with a Toolkit of Dual-Marker Selection Cassettes
PMID: 26232410 PMCID: PMC4596661 DOI: 10.1534/genetics.115.180679

we created a dual-marker cassette containing both a reporter expressing green fluorescent protein (GFP) in the pharynx (Pmyo-2::GFP::unc-54 3′UTR) and a neomycin resistance transgene (Prps-27::neoR::unc-54 3′UTR). These two marker transgenes were flanked with loxP sites and two restriction enzyme sites for convenient insertion of homology arms of interest (Figure 1B).